Simultaneous or even sequential vaccination against different variants would likely provide enhanced protection against potential further forms of the coronavirus
Jakob Kreye
The findings of the Berlin scientists are based on an analysis of antibodies that they isolated from the blood of 40 individuals. All patients had been infected with the Beta variant of SARS-CoV-2. Of the approximately 300 antibodies initially surveyed, 81 bound particularly strongly to the spike protein of the coronavirus. Dr. Reincke and his colleagues unraveled the genetic blueprints of the antibodies. This enabled them to understand which genes play a role in the assembly of these antibodies and to artificially produce these immune proteins for further studies. In doing so, they benefited from a project funded by the Helmholtz Association: the ‘BaoBab Innovation Lab’. Within the framework of BaoBab, they develop and refine technologies for the characterization and production of antibodies. “We tested if antibodies against the Beta variant are also effective against other virus variants. This is called cross-reactivity. Our analyses show that some of these antibodies do little against the wild type. Others, however, are very effective against the original virus strain and also against some of the Variants of Concern. Some of the antibodies against Beta are even effective against the currently circulating variants Delta and Omicron,” says Dr. Jakob Kreye, last author of the study and a scientist at Charité’s Department of Pediatric Neurology and the Department of Neurology with Experimental Neurology as well as DZNE.
The key to cross-reactivity is the site of the spike protein a particular antibody binds to and whether this site has changed between viral variants. “The antibodies with broad efficacy target areas of the spike protein that have remained largely the same in the previous viral variants,” Dr. Kreye says. However, there are exceptions to this in the case of Omicron. “Yet we have found antibodies that work well against both Beta and Omicron and only weakly against other variants. These particular antibodies bind to sites of the spike protein that are quite similar in Beta and Omicron but not in other variants.”
Cross-reactivity could prove to be an important aspect of future vaccinations: “Individual antibodies against the wild type also have broad efficacy. This has been described in the literature and is also shown by studies in our laboratory. Taking together these data and our current findings, we conclude that antibodies generated against different viral variants can complement each other and thus jointly improve the potency of the immune response against emerging variants. Largest possible diversity in the antibody response seems to make sense,” says Prof. Dr. Harald Prüss, senior physician at the Department of Neurology with Experimental Neurology on Campus Charité Mitte and research group leader at DZNE. Dr. Kreye adds: “Simultaneous or even sequential vaccination against different variants would likely provide enhanced protection against potential further forms of the coronavirus. This approach could be relevant for the further development of vaccination strategies, because the pathogen can be expected to keep changing in the future.”
Source: Charité – Universitätsmedizin Berlin / Deutsches Zentrum für Neurodegenerative Erkrankungen
Source: Healthcare in Europe
